Aberrant methylation of tumour-related genes in thymic epithelial tumours

Hirose, Yukiko; Kondo, Kazuya; Takizawa, Hiromitsu; Nagao, Taeko; Nakagawa, Yasushi; Fujino, Haruhiko; Toba, Hiroaki; Kenzaki, Koichiro; Sakiyama, Shoji; Tangoku, Akira

doi:10.1016/j.lungcan.2008.07.015

« Previous Next »Lung Cancer
Volume 64, Issue 2 , Pages 155-159, May 2009

Aberrant methylation of tumour-related genes in thymic epithelial tumours

Yukiko Hirose
- Department of Oncological and Regenerative Surgery, Institute of Health Biosciences, The University of Tokushima Graduate School, Japan
Kazuya Kondo
- Department of Oncological Medical Services, Institute of Health Biosciences, The University of Tokushima Graduate School, 18-15 Kuramoto-cho 3, Tokushima 770-8509, Japan
- Corresponding author. Tel.: +81 88 633 9031; fax: +81 88 633 9031.
Hiromitsu Takizawa
- Department of Oncological and Regenerative Surgery, Institute of Health Biosciences, The University of Tokushima Graduate School, Japan
Taeko Nagao
- Department of Oncological and Regenerative Surgery, Institute of Health Biosciences, The University of Tokushima Graduate School, Japan
Yasushi Nakagawa
- Department of Oncological and Regenerative Surgery, Institute of Health Biosciences, The University of Tokushima Graduate School, Japan
Haruhiko Fujino
- Department of Oncological and Regenerative Surgery, Institute of Health Biosciences, The University of Tokushima Graduate School, Japan
Hiroaki Toba
- Department of Oncological and Regenerative Surgery, Institute of Health Biosciences, The University of Tokushima Graduate School, Japan
Koichiro Kenzaki
- Department of Oncological and Regenerative Surgery, Institute of Health Biosciences, The University of Tokushima Graduate School, Japan
Shoji Sakiyama
- Department of Oncological and Regenerative Surgery, Institute of Health Biosciences, The University of Tokushima Graduate School, Japan
Akira Tangoku
- Department of Oncological and Regenerative Surgery, Institute of Health Biosciences, The University of Tokushima Graduate School, Japan

Received 22 May 2008; received in revised form 29 July 2008; accepted 30 July 2008. published online 09 September 2008.

Summary

Background

Thymoma is an uncommon neoplasm derived from epithelial cells of the thymus. Few studies have addressed the genetic alterations that occur in the tumourigenesis of thymoma.

Methods

We examined aberrant DNA methylation of DAP-K, p-16, MGMT and HPP1 genes in 26 thymomas and 6 thymic carcinoma to clarify the association between aberrant DNA methylation and clinicopathological features.

Results

Fifteen (47%) of 32 thymic epithelial tumours showed aberrant methylation. Aberrant methylation was more frequent in thymic carcinoma (86%) than in thymoma (29%). Moreover, the frequency of tumours with methylation of multiple genes in thymic carcinoma was higher than in thymoma (60% vs 20%). In thymoma, the frequency of tumour methylation, including the type A tumour component (28%), was lower than that of tumours with type B tumour component (42%). MGMT methylation was detected in 23% of thymoma and in 83% of thymic carcinoma. The frequency of methylation of the MGMT gene in both tumours was high compared with the other 3 genes.

Conclusions

Aberrant DNA methylation was more frequent in thymic carcinoma than in thymoma, and the frequency of DNA methylation in thymic epithelial tumours is roughly parallel to their malignant behaviour.

Keywords: DNA methylation, Cancer-related genes, O⁶-methylguanine-DNA methyltransferase, Thymoma, Thymic carcinoma